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	Select two accessions Accession 1: Accession 2: Select the region of interest Chromosome Start position: End position: PCR conditions options Oligos (nM): MgCl2 (mM): Monovalent cation (mM): dNTPs (mM): Primer design options Tm max: Tm min:Max Tm difference: GC% max: GC% min:
References: Nemri et al. (submitted). Marker development for the genetic study of natural variation in Arabidopsis thaliana.

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